Search results for "Proof of concept"
showing 10 items of 41 documents
Mediterranean diet and sars-cov-2 infection: Is there any association? a proof-of-concept study
2021
The aim of this observational study was investigating the possible correlation between adherence to the Mediterranean diet (MeD) and SARS-COV-2 infection rates and severity among healthcare professionals (HCPs). An online self-administrated questionnaire (evaluating both MeD adherence and dietary habits) was filled out by HCPs working in Piedmont (Northern Italy) from 15 January to 28 February 2021. Out of the 1206 questionnaires collected, 900 were considered reliable and analyzed. Individuals who reported the SARS-COV-2 infection (n = 148) showed a significantly lower MeD score, with a lower adherence in fruit, vegetables, cereals, and olive oil consumption. In a logistic regression model…
2017
Despite rapid progress, many problems and limitations persist and limit the applicability of gene-editing techniques. Making use of meganucleases, TALENs, or CRISPR/Cas9-based tools requires an initial step of pre-screening to determine the efficiency and specificity of the designed tools. This step remains time consuming and material consuming. Here we propose a simple, cheap, reliable, time-saving, and highly sensitive method to evaluate a given gene-editing tool based on its capacity to induce chromosomal translocations when combined with a reference engineered nuclease. In the proposed technique, designated engineered nuclease-induced translocations (ENIT), a plasmid coding for the DNA-…
Molecular similarities and differences from human pulmonary fibrosis and corresponding mouse model: MALDI imaging mass spectrometry in comparative me…
2017
Animal models can reproduce some model-specific aspects of human diseases, but some animal models translate poorly or fail to translate to the corresponding human disease. Here, we develop a strategy to systematically compare human and mouse tissues, and conduct a proof-of-concept experiment to identify molecular similarities and differences using patients with idiopathic pulmonary fibrosis and a bleomycin-induced fibrosis mouse model. Our novel approach employs high-throughput tissue microarrays (TMAs) of humans and mice, high-resolution matrix-assisted laser desorption/ionization-Fourier transform-ion cyclotron resonance-mass spectrometry imaging (MALDI-FT-ICR-MSI) to spatially resolve ma…
Developing an Analytical Framework for Analyzing and Comparing National E-Government Strategies
2020
Part 1: E-Government Foundations; International audience; Across the world, e-government strategies are developed for the effective digitalization of the public sector. They offer governments a framework for dealing with challenges such as technical and legal interoperability and collaboration between public and private sector stakeholders, and for promoting a future vision for a digital public sector. Since e-government strategies are policy document and, thus, likely to convey biased perspectives, analyses of these strategies can yield insights into these biases and different perspectives on public sector digitalization. Until now, there has been no widely recognized framework for systema…
A Novel Anti-CD40 Monoclonal Antibody, Iscalimab, for Control of Graves Hyperthyroidism—A Proof-of-Concept Trial
2019
Abstract Context The CD40-CD154 co-stimulatory pathway plays an important role in the pathogenesis of Graves disease (GD) by promoting autoreactive B-cell activation. Objective Evaluate efficacy and safety of a human, blocking, nondepleting anti-CD40 monoclonal antibody, iscalimab, in hyperthyroid patients with GD. Design Open-label, phase II proof-of-concept study. Setting Multicenter. Patients Fifteen with GD. Intervention Patients received 5 doses of iscalimab at 10 mg/kg intravenously over 12 weeks. Main outcome measures Thyroid-related hormones and autoantibodies, plasma soluble CD40, free CD40 on B cells, soluble CXCL13, pharmacokinetics, and safety were assessed. Results The iscalima…
Multimodal Device for Real-Time Monitoring of Skin Oxygen Saturation and Microcirculation Function
2019
The present study introduces a recently developed compact hybrid device for real-time monitoring of skin oxygen saturation and temperature distribution. The prototype involves a snapshot hyperspectral camera, multi-wavelength illuminator, thermal camera, and built-in computer with custom-developed software. To validate this device in-vivo we performed upper arm vascular occlusion on eight healthy volunteers. Palm skin oxygen saturation maps were analyzed in real-time using k-means segmentation algorithm and two-layer optical diffuse model. The prototype system demonstrated a satisfying performance of skin hyperspectral measurements in the spectral range of 507&ndash
Fast Available Bandwidth sampling for ADSL links: rethinking the estimation for larger-scale measurements
2009
Most existing tools for measuring the end-to-end available bandwidth require access to both end-hosts of the measured path, which severely restricts their usability. Few tools have been developed to overcome this limitation, but all of them focus on achieving high precision and are not suitable for large campaigns. In this paper we develop FAB-Probe , a tool aimed at characterizing the available bandwidth of a large number of paths, adapting it particularly for ADSL settings. FAB-Probe is an evolution of ABwProbe , a tool that estimates the available bandwidth in non-cooperative ADSL environments. Analyzing carefully the needs of such a characterization tool, we optimize and rethink ABwProb…
Self-Powered IoT Device for Indoor Applications
2018
This paper presents a proof of concept for selfpowered Internet of Things (IoT) device, which is maintenance free and completely self-sustainable through energy harvesting. These IoT devices can be deployed in large scale and placed anywhere as long as they are in range of a gateway, and as long as there is sufficient light levels for the solar panel, such as indoor lights. A complete IoT device is designed, prototyped and tested. The IoT device can potentially last for more than 5 months (transmission interval of 30 seconds) on the coin cell battery (capacity of 120mAh) without any energy harvesting, sufficiently long for the dark seasons of the year. The sensor node contains ultra-low pow…
BIO-Bragg gratings: structured molecular networks for on-fiber bioanalysis
2021
The research on photonic biosensors is a scientific hot topic at the moment, with a significant potential impact on industry and medicine. Label-free, miniaturized, inexpensive and low-loss biosensors are developed based on optical fiber technology. Our approach is based on a Bio-Bragg-Grating (BBG) patterned on the surface of a microfiber. We present the design, fabrication and proof of concept of our device, as well as its multiplexing and tunability perspectives [1] .